Professor, Biochemistry North-West University, South Africa
Title: Cannabis sativa induces cell cycle arrest and apoptosis in MCF-7 and MDA-MB-231 breast cancer cells
Prof Motadi is an expert in African Traditional medicine focusing mainly on it Biochemical impact on cancer development and progression. For his life career on cancer his focus has always been targeting the cellular machinery and restoring them in cancer as a way to control cancer progression.
Breast cancer incidence rate has increased beyond that of lung cancer, making it the most common malignancy among women. Breast tumour progression is partly as a result of p53 inactivation by overexpressed ubiquitous regulatory proteins that possess p53 binding domain. Restoration using any molecules of p53 might assist in the treatment and improved prognosis of breast cancer. The aim of the study was to investigate the effects of Cannabis sativa butanolic plant extract on MCF-7 and MDA-MB-231 breast cancer cell line. MTT assay, xCELLigence assay, flow cytometry, confocal microscopy, and caspase-3/7 assay were conducted to assess for anti-breast cancer activity. Cannabis sativa was able to induce cell death and inhibit cell growth MTT and xCELLigence assay at a concentration of 150µg/ml. Flow cytometry confirmed the induction of apoptosis following G0 cell cycle arrest in MCF-7 and G2/M arrest in MDA-MB-231 cells. Confocal microscopy further showed the induction of cell death, with or without caspase 3/7 activation. In conclusion, Cannabis sativa butanolic extract has the potential to induce apoptosis in MCF-7 cells and MDA-MB-231.